Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster
نویسندگان
چکیده
منابع مشابه
Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A.
Recent developments in next-generation sequencing technologies have brought recognition of microbial genomes as a rich resource for novel natural product discovery. However, owing to the scarcity of efficient procedures to connect genes to molecules, only a small fraction of secondary metabolomes have been investigated to date. Transformation-associated recombination (TAR) cloning takes advanta...
متن کاملDirect cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the potent antibiotic taromycin
1. Experimental methods 2. Strains and plasmids used in this study (Table S1) 3. Deduced functions and sequence comparison of the tar genes (Table S2) 4. Schematic diagram of the tar gene cluster direct cloning (Figure S1) 5. Restriction mapping of pCAP01-tar and its derivatives (Figure S2). 6. Results of heterologous expression of the tar gene cluster in S. coelicolor M1146 (Figure S3) 7. Resu...
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Actinoplanes friuliensis produces the lipopeptide antibiotic friulimicin, which is a cyclic peptide with one exocyclic amino acid linked to a branched-chain fatty acid acyl residue. The structural relationship to daptomycin and the excellent antibacterial performance of friulimicin make the antibiotic an attractive drug candidate. The complete friulimicin biosynthetic gene cluster of 24 open re...
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The structure of a new antifungal antibiotic, mulundocandin, C48H77N7O16, was elucidated by high field NMR experiments e.g., homo- and heteronuclear correlation spectra, distortionless enhancement by polarization transfer (DEPT) spectra as well as nuclear Overhauser effect. The compound is a lipopeptide antibiotic belonging to the echinocandin class.
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 2017
ISSN: 0021-8820,1881-1469
DOI: 10.1038/ja.2017.146